This isothermal platform can exponentially amplify DNA at a constant temperature using a polymerase and nicking enzyme in the temperature range from 55 °C to 59 °C. The potential benefits of NEAR over PCR are increased speed and lower energy requirements. This platform can be used in bedside diagnostic products and delivers results quickly in minutes.
Picture from: Niemz A, Ferguson TM, Boyle DS. Point-of-care nucleic acid testing for infectious diseases. Trends Biotechnol. 2011 May;29(5):240-50.